![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K1583101:Design
Designed by: Hector Sanguesa Ferrer, Max van Group: iGEM15_TU_Delft (2015-09-04)
pRha + CsgA + His-tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The nucleotides encoding for the second and third amino acid of the CsgA gene were changed to optimize synthesis success. (silent mutations)
Source
The DNA was synthesized. The sequence of CsgA originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055). The design was based on the paper ""Programmable biofilm-based materials from engineered curli nanofibres""[1]
1. Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5.